Workshop on Molecular microbiology

Basic microbiology techniques : Gram staining of bacteria, preparation of culture media, Isolation and enumeration of microorganism from soil by the serial dilution agar plating method, methods of obtaining pure culture of microorganisms, maintenance of pure culture.

Basic molecular biology techniques: primer design method and PCR operation demo, Plasmid isolation, agarose gel electrophoresis

 

Workshop of Fermentation Technology

Media optimization using placket burman design, preparation of bioreactor and utilities of bioreactor operation, evaluation of growth, substrate and product characteristics of microorganisms, immobilization of yeast cell by gel entrapment using sodium alginate, effect of substrate concentration on kinetics of invertase enzyme, simulation in Matlab.

 

Workshop on Animal cell culture

Basics of cell culture, Culture environment, Protocol for sub-culturing of adherent cells, Protocol for sub-culturing of suspension cells, Cell counting, Cryopreservation of cells

 

Workshop on Molecular techniques in cancer diagnosis

Immunohistochemistry, Western Blot, Polymerase chain reaction

 

 Workshop on Techniques in Drug discovery

Isolation of active compounds, Anti proliferative assays, cytotoxicity studies, identification of mechanisms by PCR and Western blot

 

Workshop on flow cytometer

Introduction (Principle & Theory), Instrumentation, Fluorescence and Flurochrome used in flowcytometer, DNA analysis, Viability assessment of bacteria model and Data analysis.

 

Quiz and panel discussion

 

Instrument Demo: HPLC, Flow cytometer, PCR, RT-PCR, AFM, Bioreactor, FPLC.

 

Instrument Demonstrations

 

• COD measurement of wastewater
• BOD measurement of wastewater
• Ion Chromatograph
• Atomic Absorption Spectrophotometer
• UV-Vis Spectrophotometer
• Gas Chromatograph
• Kjeldahl’s Nitrogen Analyser
• Fourier Transform Infrared Spectrophotometer (FTIR)
• Fluorescence Microscope
• Flame Photometer
• High Performance Liquid Chomatography (HPLC)
• Fluorescence Spectrophotometer
• PCR
• Biotech Hub